MASSIVE ANALYSER

Massive Analyser provides a safe, functional, and easily accessible cloud-based platform to analyse and organise genomic data. Our platform supports various NGS platforms, Kits, files as input, and many more features to come. Massive Analyser gives all necessary metrics, annotated variants, related database links, copy number variations, ACMG based classifications, phenotype information, pathogenicity evaluation, allele frequencies, variation consequences, and integrated visualization in one simple page. Since each specialist might have different priorities during the analysis, our platform supports multiple presets for page configuration and filtering options in order to give clinicians more elasticity.

FEATURES

Massive Analyser is a platform designed to enrich the user experience to the fullest by utilizing customizable features.

Detects Germline and Somatic Variants from FASTQ, BAM, and VCF Files
Automatically Classifies the Pathogenicity of Variants According to ACMG Guideline
Detects de novo Variants with Family Trio Analyses
Detects the Copy Number Variants
Gives the Statistical Information Per Sample
Automatically Generate Clinical Reports with Massive Analyser Reporting System

JUST UPLOAD YOUR SAMPLE INPUT AND CHOOSE THE TYPE OF ANALYSIS

Our platform currently contains 8 different pipelines which 3 of them are the main pipelines (Exome, Trio, Advance CNV Detector). The exome pipeline has 3 sub-pipelines that have different starting points and each of the sub-pipelines have two options as somatic or germline. The number of pipelines will be increased over time to fulfill the arising clinical needs.

TECH SPECS

Upload NGS output directly to our platform without the need for any manipulation.
ISO/IEC 27001:2013, ISO9001:2015, ISO 14001:2015, ISO 45001:2018, ISO13485
GDPR and KVKK compliance
Technical support
Suitable for patient analysis and report
FASTQ, BAM and VCF files are accepted as input. (VCF files should be in our format)
Variant fraction (as Genomic Fraction Coverage), zygosity status and more statistics are given.
CES and WES data analysis and review includes phenotype specific variant filter.
Analysis done according to ACMG variant classification criteria. (The exome and trio analyses provide, 19 and 20 of the proofs automatically to determine the pathogenicity class of variants.)
Graph and charts are given for the following; (and more)
1. zygosity
2. chromosomal position
3. effects on the transcript
4. automatic pathogenicity calculation
5. sequence onthology information (frameshift, missense etc.)
6. dbSNP number
7. Clinvar records
8. Gene symbol and gene symbol related links
PolyPhen / SIFT / Mutation Taster and DANN Revel / MetalR and many more variant simulators are supported.
ExAC / ESP / 1000 Genomes /Gnomad are used for the population analysis of the variant.

From FASTQ to WEB Report!

Just Upload Your Input and Let Our App Work Its Magic

Massive Analyser

Contact Us for Details

Built-in IGV

Access IGV by clicking on a variant in the CNV table!

Customizable Features

Customize your own variant and statistics table and apply filters of your choice from the advanced settings menu! You can also customize pathogenicity and rules columns and many more…

Detailed Charts & Graphs

Even for Advanced CNV and Trio Analyses!

Copy of EXOME PANEL OUTLINE for KeremY. (1)

Not Just from FASTQ

You can upload BAM and VCF as input too! (VCF should be uploaded in our format)

Automatically Generated Medical Report

Just hit the button!

Access it from Anywhere with an Internet Connection!

SAMPLE FIGURES

Main Statistics

This page includes basic statistics of the aligned data such as globals, coverage, GC-content, gene-based coverage, mapping quality, mismatches and indels, insert size, zygosity ratio, tumor mutational burden, Ts-Tv ratio.

CNV Diagram

The diagram shows chromosome based copy number variants. While dark blue denotes loss, dark red denotes gain.

CNV Table & Plots

The scattered plot visualizes the base 2 logarithm of copy ratio via chromosomes. Bands below a certain threshold below zero mean loss, while bands above a threshold value above zero mean gain. Dots denote trustability of detected CNV. If dot density is high and dot diameters large, reability of the result is also acceptable. To be sure for about reliability of results, please check the CNV weight and log2 value from CNV table.

Genomic Fraction Coverage

In each depth of coverage, the graph shows percentage of reads that covered in that depth.

$gen_frac_cov$

Chromosome-Based Copy Ratio

The chart visualizes the base 2 logarithm of copy ratio via chromosomes. While bands less than zero means deletion, bands greater than zero mean duplication. The amount of duplication and deletion increases as bands move away from zero.