SSu – LSu Metagenomics Analyses
Metagenomics analyzes make possible to determine the diversity and amounts of microorganisms in a sample. Identification of bacteria with the 16S rRNA gene with a length of ~ 1500 bp is now a standardized process. However, most of the new generation sequencing methods used only a portion of this gene which is approximately ~ 450 bp in length and therefore produce erroneous results. Oxford Nanopore enhanced the resolution of species identification by reading the entire 1500 bp 16S rRNA gene thanks to its long reading technology and opened a new page in this field. Metagenomic analyzes provide a large number of possibilities for detailed examination of a wide variety of sample types, or components of a sample.
Massive Bioinformatics offers standardized metagenomic analyzes using Illumina systems as well as long amplicons produced with Oxford Nanopore technology. It is possible to increase taxonomic resolution and achieve more accurate results by amplifying the entire 16s rRNA region of about 1500 bp length as well as analysis with a hypervariable region of about 450 bp length including the V3-V4 region of the 16s rRNA region.
Metagenomic analyzes of bacteria and archae groups with 16s rRNA gene, fungi with ITS region, and eukaryotic organisms with 18S rRNA gene are performed by using barcode genes for different living groups. It is possible to use different databases according to the content of the study, combinations with different workflows to increase the accuracy of the results are among the services we provide.
In population and biomarker studies, comparative analysis is performed between samples and related visual graphics are created in accordance with your study.
| Sample Size | Platform | Analysis*** | Price(Per sample)** |
|---|---|---|---|
| 1-12 | Oxford Nanopore | Bioinformatics Analyzes* | Contact Us |
| 13-48 | Oxford Nanopore | Bioinformatics Analyzes* | Contact Us |
| 48- | Oxford Nanopore | Bioinformatics Analyzes* | Contact Us |
* Prices for analyzes with Oxford Nanopore do not include sequencing fees.
** VAT is not included in the prices.
*** The DNA of the samples to be sent for sequencing must be isolated and comply with the following parameters:
DNA should be checked by Agarose gel electrophoresis to confirm no degradation
The total amount should be 2 μg and above with a concentration of 20 ng / μl and above
Bioinformatics Analyzes
Bioinformatics Analyzes
Species Diversity and Quantity Analysis
Quantitative determination and demonstration of species distributions in the sample
Comparative Taxonomic Analysis
Quantitative determination and comparison of microbial diversity in samples
Principal Coordinate and Component Analyzes
Performing principal coordinate analysis together with other parameters to reduce variation dimentions
Alpha – Beta Diversity Analyzes
Demonstrate how taxonomic diversity and richness are distributed within the samples by alpha diversity and how the taxonomic diversity is distributed between different samples by beta diversity
